Effect of droplet splash on the dispersal of soil-borne Corynespora cassiicola cusing cucumber leaf spot
Cucumber leaf spot caused by Corynespora cassiicola is an important disease in cucumber produc-tion,and diseased residues in soil have been proved to be the main source of its initial infection.To clarify the effect of droplet splash on the dispersal of the pathogen,a real-time fluorescence quantitative PCR(qPCR)-based system for rapid detection of C.cassiicola in soil was established.Through this system,C.cassiicola was detected in soil samples collected from greenhouses where Corynespora leaf spot was naturally occurred,with concentrations ranging from 27.00×100 to 7.95×105 pg DNA·g-1.The results of artificial simulation of droplet splashing experiment showed that the impact of droplets carrying C.cassiicola led to splash dispersal of the pathogen and increased disease severity,with disease index of 65.56 and 41.56 for C.cassiicola-infected soil and substrate,respectively.Mulching soil and substrate surface in the above experiment significantly reduced disease severity,with disease index of 13.79 and 13.83 for C.cassiicola-infected soil and substrate,respectively.The re-sults of this study indicate that droplet splash is an important route for the transmission of soil-borne C.cassiico-la,providing not only technical assistance for monitoring and early warning of Corynespora leaf spot but also theoretical basis for the prevention and control of the disease.
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