Identification of an endophytic Pantoea ananatis strain XP-1 with antagonistic effect to Xanthomonas oryzae pv.oryzicola and growth-promoting effect on rice seedlings
Rice bacterial leaf streak,the main bacterial disease caused by Xanthomonas oryzae pv.oryzicola(Xoc),seriously affects rice production.Exploring safe and efficient biological resources is very important for the control of the disease.In this study,an endophytic bacterial strain XP-1 was obtained from rice leaves by confronting incubation method,with an inhibition zone diameter of(56.1±0.1)mm against Xoc.XP-1 also exhibited good antagonistic activity against 5 common bacterial phytopathogens(Ralstonia solanacearum,Xanthomonas oryzae pv.oryzae,Ralstonia solanacearum,Pectobacterium carotovorum subsp.carotovorum,Xanthomonas fragariae and Xanthomonas campestris pv.campestris).Physiological and biochemical test showed that XP-1 strain can produce IAA and siderophore,and solubilize phosphate but cannot solubilize potassium and fix nitrogen.Through indoor seed soaking test and greenhouse pot experiment,it was showed that the diluted fermentation broth of XP-1 could promote seed germination and seedling growth of rice plants,and inhibit leaf lesion on 3 rice varieties,with an inhibition rate of 63.72％-94.47％.The beneficial endophytic XP-1 strain,which has a wide antimicrobial spectrum,was identified as Pantoea ananatis based on morphological characteris-tics,physiological and biochemical properties and molecular identification results.This study provides the foundation for further exploration of biological resources for rice bacterial leaf streak control.
endophytic bacteriaPantoea ananatisidentificationbacterial leaf streak of ricedisease prevention and growth promotion
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