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粗叶榕茎枯病病原菌的分离与鉴定

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Isolation and identification of the pathogen causing stem blight of Ficus hirta
Ficus hirta(vahl)is a valuable medicinal plant as well as edible plants in southern China.In 2022,a new stem blight disease was observed to infect Ficus hirta(vahl)in Danzhou of Hainan province.The inci-dence of disease reached 47.5％in fields.In order to clarify the pathogen of Ficus hirta stem blight disease,ten isolates with the same morphology were isolated from blight tissue by using a conventional tissue separation method.MA2,a representative strain with excellent growth,was selected for pathogenicity test on Ficus hirta plants,observation of morphological characteristics,sequence analysis on ITS,TUB and TEF,and phylogenetic tree construction.The results showed that morphological characteristics of the isolate MA2 was similar to that of Lasiodiplodia pseudotheobromae.The isolate MA2 was clustered into one clade in phylogenetic tree with L.pseudotheobromae.The above results indicated that the stem blight of F.hirta was caused by L.pseudotheobro-mae.This is the first report of a stem blight disease on F.hirta in China was identified as L.pseudotheobromae.

Ficus hirtastem blightLasiodiplodia pseudotheobromaepathogen identification

贺春萍、胡月、吴贺、梁艳琼、李锐、林金晶、陆英、吴伟怀、易克贤

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中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室,海口 571101

华中农业大学植物科学技术学院,武汉 430070

云南农业大学农学与生物技术学院,昆明 650201

中国热带农业科学院三亚研究院,三亚 572025

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Ficus hirta stem blight Lasiodiplodia pseudotheobromae pathogen identification

海南省院士创新平台科研专项海南省自然科学基金国家天然橡胶产业技术体系建设项目病害防控岗位项目

YSPTZX202016320RC692CARS-33-BC1

2024

10.13926/j.cnki.apps.000892

植物病理学报
中国植物病理学会

植物病理学报

CSTPCD北大核心
影响因子：0.865
ISSN：0412-0914
年,卷(期)：2024.54(4)