首页|侵染浙江省和山东省西瓜的西瓜银灰斑驳病毒的鉴定

侵染浙江省和山东省西瓜的西瓜银灰斑驳病毒的鉴定

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Identification of watermelon silver mottle virus on watermelon in Zhejiang and Shandong provinces
During the disease survey in spring of 2023,watermelon in Shandong province and Zhenjiang prov-ince displayed silver grey and irregular mottled symptoms on leaves.In order to identify the virus,ten samples including the leaves,stem and the young fruit were collected,and total RNA was extracted from each sample.The RT-PCR method was used to amplify the conserved partial N gene sequence encoded by watermelon silver mottle virus(WSMoV)S RNA,and the partial N gene was cloned and sequenced.Sequence analysis showed that the sequence identity of WSMoV-Zhejiang and WSMoV-Shandong isolates with WSMoV-Thailand SN858 and WSMoV-Yunnan Banna-2011 isolates was over 99.0％.Furthermore,phylogenetic analysis showed that the partial N gene clustered with the Thailand and Yunnan-Banna-2011 isolates.The results showed that watermelon silver mottle virus(WSMoV)occurred in watermelon in Zhejiang and Shandong provinces.This is the first time the virus has infected watermelon in Zhejiang and Shandong provinces.

watermelon silver mottle virus(WSMoV)watermelonShandong provinceZhejiang prov-incesequence analysis

吴会杰、严蕾艳、王驰、孙建磊、王崇启、高超、康保珊、刘莉铭、刘茜、古勤生

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中国农业科学院郑州果树研究所,郑州 450009

宁波市农业科学院蔬菜研究所,宁波 315040

温岭市农业农村和水利局,温岭 317500

山东省农业科学院蔬菜研究所,济南 250100

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watermelon silver mottle virus(WSMoV) watermelon Shandong province Zhejiang prov-ince sequence analysis

现代农业产业技术体系建设项目中国农科院科技创新工程项目

CARS-25-2023-G20CAAS-ASTIP-2022-ZFRI-09

2024

10.13926/j.cnki.apps.001334

植物病理学报
中国植物病理学会

植物病理学报

CSTPCD北大核心
影响因子：0.865
ISSN：0412-0914
年,卷(期)：2024.54(4)
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