侵染浙江省和山东省西瓜的西瓜银灰斑驳病毒的鉴定
Identification of watermelon silver mottle virus on watermelon in Zhejiang and Shandong provinces
吴会杰 1严蕾艳 2王驰 3孙建磊 4王崇启 4高超 4康保珊 1刘莉铭 1刘茜 1古勤生1
作者信息
- 1. 中国农业科学院郑州果树研究所,郑州 450009
- 2. 宁波市农业科学院蔬菜研究所,宁波 315040
- 3. 温岭市农业农村和水利局,温岭 317500
- 4. 山东省农业科学院蔬菜研究所,济南 250100
- 折叠
Abstract
During the disease survey in spring of 2023,watermelon in Shandong province and Zhenjiang prov-ince displayed silver grey and irregular mottled symptoms on leaves.In order to identify the virus,ten samples including the leaves,stem and the young fruit were collected,and total RNA was extracted from each sample.The RT-PCR method was used to amplify the conserved partial N gene sequence encoded by watermelon silver mottle virus(WSMoV)S RNA,and the partial N gene was cloned and sequenced.Sequence analysis showed that the sequence identity of WSMoV-Zhejiang and WSMoV-Shandong isolates with WSMoV-Thailand SN858 and WSMoV-Yunnan Banna-2011 isolates was over 99.0％.Furthermore,phylogenetic analysis showed that the partial N gene clustered with the Thailand and Yunnan-Banna-2011 isolates.The results showed that watermelon silver mottle virus(WSMoV)occurred in watermelon in Zhejiang and Shandong provinces.This is the first time the virus has infected watermelon in Zhejiang and Shandong provinces.
关键词
watermelon silver mottle virus(WSMoV)/watermelon/Shandong province/Zhejiang prov-ince/sequence analysisKey words
watermelon silver mottle virus(WSMoV)/watermelon/Shandong province/Zhejiang prov-ince/sequence analysis引用本文复制引用
基金项目
现代农业产业技术体系建设项目(CARS-25-2023-G20)
中国农科院科技创新工程项目(CAAS-ASTIP-2022-ZFRI-09)
出版年
2024
NETL
NSTL
万方数据