Abstract
To clarify the species and biological characteristics of the pathogenic fungi that cause root rot of Ficus virens in Hainan Province,pathogenic fungi were isolated from diseased tissue and basidiocarps collected from the root of the diseased tree of Ficus virens in March 2022 by tissue separation method.The pathogenic bac-teria causing the root rot disease were identified through pathogenicity determination,combing with morphology identification and molecular identification.The effects of pH,temperature,medium and light on mycelium growth under different treatments were measured by cross crossing method.The results showed that the isolated pathogenic ganoderma strain was the pathogen causing the root rot of Ficus virens.After morphological charac-terization and jointly phylogenetic tree construction based on rDNA-ITS,rDNA-LSU and rDNA-SSU,the patho-gen strain was identified as Ganoderma tropicum.The biological characteristics showed that the optimal medium was PDA medium,the optimal pH was 7.0,the optimal light condition was total darkness,and the optimal growth temperature was 32 ℃.It will provide scientific theoretical basis for the diagnosis and control of this disease.
维普
万方数据