首页|Functional significance of asymmetrical retention of parental alleles in a hybrid pine species complex

Functional significance of asymmetrical retention of parental alleles in a hybrid pine species complex

扫码查看
原文链接
  • 万方数据
  • 维普
Hybrid genomes usually harbor asymmetrical parental contributions.However,it is challenging to infer the functional significance of asymmetrical retention of parental alleles in hybrid populations of conifer trees.Here we investigated the diversity in the glutathione S-transferase(GST)gene family in a hybrid pine Pinus densata and its parents(Pinus tabuliformis and Pinus yunnanensis).Plant GSTs play major roles in protecting plants against biotic and abiotic stresses.In this study,19 orthologous groups of GST genes were identified and cloned from these three species.We examined their expression in different tissues,and then purified the corresponding proteins to characterize their enzymatic activities and specificities toward different substrates.We found that among the 19 GST orthologous groups,divergence in gene expression and in enzymatic activities toward different substrates was prevalent.P.densata preferentially retained P.yunnanensis-like GSTs for 17 out of the 19 gene loci.We determined the first GST crystal structure from conifer species at a resolution of 2.19 Å.Based on this structure,we performed site-directed mutagenesis to replace amino acid residuals in different wild-types of GSTs to understand their functional impacts.Reciprocal replacement of amino acid residuals in native GSTs of P.densata and P.tabuliformis demonstrated significant changes in enzyme functions and identified key sites controlling GSTs activities.This study illustrates an approach to evaluating the functional significance of sequence variations in conifer genomes.Our study also sheds light on plausible mechanisms for controlling the selective retention of parental alleles in the P.densata genome.

enzymatic functionfunctional divergencegene expressionglutathione S-transferaseshomoploid hybrid species

Chang Qu、Hong-Na Kao、Hui Xu、Bao-Sheng Wang、Zhi-Ling Yang、Qi Yang、Gui-Feng Liu、Xiao-Ru Wang、Yan-Jing Liu、Qing-Yin Zeng

展开 >

State Key Laboratory of Tree Genetics and Breeding,Chinese Academy of Forestry and Northeast Forestry University,Beijing 100091,China

Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China

Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Menglun,Yunnan 666303,China

State Key Laboratory of Subtropical Silviculture,College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou,Zhejiang 311300,China

Department of Ecology and Environmental Science,UPSC,Umeå University,SE-90187 Umeå,Sweden

展开 >

Fundamental Research Funds for the Central Nonprofit Research Institution of the Chinese Academy of ForestryFundamental Research Funds for the Central Nonprofit Research Institution of the Chinese Academy of ForestryFundamental Research Funds for the Central Nonprofit Research Institution of the Chinese Academy of ForestryFundamental Research Funds for the Central Nonprofit Research Institution of the Chinese Academy of Forestry

CA-FYBB2019ZY002TGB2020001TGB2021001TGB2022001

2024

10.1111/jse.12953

植物分类学报
中国科学院植物研究所　中国植物学会

植物分类学报

CSTPCD
影响因子：0.322
ISSN：1674-4918
年,卷(期)：2024.62(1)
  • 38