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Seeking the identity of an enigmatic moss by embracing phylogenomics

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Seeking the identity of an enigmatic moss by embracing phylogenomics
Currently,a wide range of genomic techniques is available at a relatively affordable price.However,not all of them have been equally explored in bryophyte systematics.In the present study,we apply next-generation sequencing to identify samples that cannot be assigned to a taxon by morphological analysis or by Sanger sequencing methods.These samples correspond to a moss with an enigmatic morphology that has been found throughout Western Europe over the last two decades.They exhibit several anomalies in the gametophyte and,on the rare occasions that they appear,also in the sporophyte.The most significant alterations are related to the shape of the leaves.Morphologically,all specimens correspond to mosses of the genus Lewinskya,and the least modified samples are potentially attributable to the Lewinskya affinis complex.Specimen identifications were first attempted using up to seven molecular markers with no satisfactory results.Thus,we employed data generated from targeted enrichment using the GoFlag 408 flagellate land plant probe set to elucidate their identity.Our results demonstrate that all the enigmatic samples correspond to a single species,L.affinis s.str.This approach provided the necessary resolution to confidently identify these challenging samples and may be a powerful tool for similar cases,especially in bryophytes.

BryophytaGoFlag 408Lewinskya affinisOrthotrichaceaetargeted enrichmenttaxonomy

Pablo Aguado-Ramsay、Tamara Villaverde、Ricardo Garilleti、J.Gordon Burleigh、Stuart F.McDaniel、Maren Flagmeier、Jurgen Nieuwkoop、Arno van der Pluijm、Florian Hans、Francisco Lara、Isabel Draper

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Departamento de Biología,Facultad de Ciencias,Universidad Autónoma de Madrid,C.Darwin,2,Madrid 28049,Spain

Doctoral Program in Biology,Doctoral School(EDUAM),Universidad Autónoma de Madrid,C.Francisco Tomás y Valiente,2,Madrid 28049,Spain

Departamento de Biología,Geología,Física y Química Inorgánica,Universidad Rey Juan Carlos,C.Tulipán s/n,Móstoles 28933,Spain

Departamento de Botánica y Geología,Facultad de Farmacia,Universidad de Valencia,Avda.Vicente Andrés Estelles s/n,Burjassot 46100,Spain

Department of Biology,University of Florida,220 Bartram Hall,Gainesville,FL 32611,Florida,USA

Vluchtheuvelstraat 6,Dreumel,BK 6621,The Netherlands

Visserskade 10,Hank,GL 4273,The Netherlands

Kreuzweiler Straße 22,Perl-Sinz D-66706,Germany

Centro de Investigación en Biodiversidad y Cambio Global,Universidad Autónoma de Madrid,C.Darwin,2,Madrid 28049,Spain

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Bryophyta GoFlag 408 Lewinskya affinis Orthotrichaceae targeted enrichment taxonomy

2024

10.1111/jse.13040

植物分类学报
中国科学院植物研究所　中国植物学会

植物分类学报

CSTPCD
影响因子：0.322
ISSN：1674-4918
年,卷(期)：2024.62(5)