两种IGRA检测方法诊断结核分枝杆菌潜伏感染的效能分析
栗方芝 1李娜娜 1石亚萍 1贺仁忠 1陈玲1
作者信息
- 1. 贵州省遵义医学院附属医院呼吸二科 呼吸医学研究室 贵州遵义 563003
- 折叠
摘要
目的：比较γ-干扰素释放试验TB-IGRA和T-SPOT.TB检测结核分枝杆菌潜伏感染的效能和一致性。方法分别用TB-IGRA和T-SPOT.TB两种方法对18例志愿者、4例涂阳肺结核患者及1例非结核分枝杆菌感染者的外周血标本中特异性γ-干扰素的含量进行检测，进行平行比较分析。结果 TB-IGRA法和T-SPOT.TB法的敏感性、特异性、阳性预测值、阴性预测值均为100%,2种方法的Youden指数均为1，差异无统计学意义(P＞0.05)。结论 TB-IGRA和T-SPOT.TB两种方法在诊断结核分枝杆菌潜伏感染方面有较好的效能和一致性，有临床应用价值，阴性结果在结核病鉴别诊断中有较重要的临床意义。
Abstract
Objective To compare efficiency and consistency of type interferon gamma release assay on detecting mycobacterium tuberculosis latent infection by two different methods of TB-IGRA and T-SPOT.TB. Method Detect the specific IFN-γ of 18 volunteers, 4 patients with smear positive pulmonary tuberculosis, 1 case of non tuberculous mycobacterial infection for paral el comparative analysis by the two different methods. Results Sensitivity, specificity, positive predictive value, and negative predictive value of TB -IGRA and T-SPOT. TB were 100%, Youden index of the two methods was 1, indicating no statistical y significant (P > 0.05). Conclusion TB-IGRA and T-SPOT.TB have good consistency in the diagnosis of mycobacterium tuberculosis latent infection with a good clinical application value, and the negative result has a valuable significance in the differential diagnosis of tuberculosis.
关键词
结核分枝杆菌/潜伏感染/γ-干扰素释放试验/TB-IGRA/T-SPOT.TBKey words
Mycobacterium tuberculosis/Latent infections/Interferon gamma release assay/TB-IGRA/T-SPOT.TB引用本文复制引用
基金项目
贵州省卫生厅科学技术基金(gzkj2009-1-009)
贵州省结核病综合防治2011协同创新中心(培育)项目()
出版年
2014
NETL
NSTL
万方数据