Objective:To investigate the effects of 2% lidocaine mortar on the fistula in maintenance hemodialysis patients.Methods:120 patients were randomly divided into 2 groups (n=60 for each). The observation group using 2% lidocaine mortar to infiltrate before the puncture,while the control group using conventional disinfection. After the treatment,the patients were evaluated for the pain using numerical rating scale and the Wong-Baker facial expressions scale respectively for a total of 4 weeks. Recording each fistula blood flow and the rate of both blocking and infection in these patients. Results: The degree of pain in the observation group was significantly lower than that in the control group (P <0.01). There were no significant difference in the fistula blood flow and the rate of both blocking and infection in the two groups.Conclusion:Using 2% lidocaine mortar to infiltrate can significantly reduce the pain of puncture,but has little effect on blood flow and infection in the maintenance hemodialysis patients.