Objectives To analyze the awareness rate of pulmonary tuberculosis among the public of Guizhou province and its influencing factors,and to provide scientific evidence for further improvement of Tuberculosis health education.Methods An awareness rate survey of 6971 persons who were in 5 sites of the fifth national tuberculosis epidemiological survey in Guizhou. Single factor analysis and multiple linear regressions were used to determine factors associated with tuberculosis messages score. Results The public awareness rate of key tuberculosis messages was 33.1%. Key tuberculosis messages scores were associated with gender, distribution of rural and urban, age, ethnic and tuberculosis medical history(P<0.01). Conclusion The awareness rate of key tuberculosis messages was lower in Guizhou province. It is necessary to focus on health education of the key crowd.