Objective To investigate the relationship between NAFLD and insulin resistance. Methods The Non-alcoholic fatty liver disease patients are divided into mild group, moderate group and severe group by the results of liver color Doppler ultrasonography. Each group were measured height, weight, blood glucose, triglycerides, cholesterol, insulin and ALT to calculate the BMI, HOMA-IR and HBCI for comparison. Results 1.Compared with control group, ALT、triglycerides, cholesterol、BMI、WHR in NAFLD groups were significantly higher (p<0.05). HOMA-IR and HBCI have significant difference in mild group, moderate group and severe group(p<0.05). Patients with NAFLD have more positive tests for HOMA-IR and HBCI than others.Conclusions Patients with NAFLD have more severe insulin resistance.And insulin resistance getting worse is associated with more severe NAFLD.