objective to investigate the serum prealbumin and bile acid test in the value of liver injury. methods The medical research to select 100 cases of our hospital from January 2009 to January 2013 were between liver system disease patients as observation object, choosing to 50 cases of medical examination at the same time as the healthy control group, comparison and analysis of different patients serum prealbumin and bile acid test results. Results different types of liver injury disease patients and the control group patients serum prealbumin (PA), bile acid (associates), glutamic acid transaminase (ALT), total bilirubin (TBIL) and albumin (propagated) test results compared with significant statistical difference (P < 0.05). Conclusion by the medical research result, serum prealbumin and bile acid are more accurate and reliable clinical diagnosis of liver injury.