目的：观察小鼠皮下注射α7烟碱型乙酰胆碱受体（α7nAchR）拮抗剂甲基牛扁亭对100%氧吸入后酵母多糖所诱导的脓毒症小鼠的变化及其意义。方法：腹腔注射酵母多糖或等容量生理盐水，随机分组为：(1)生理盐水+空气；(2)酵母多糖+空气；(3)酵母多糖+空气+甲基牛扁亭组；(4)酵母多糖+100%氧气；(5)酵母多糖+100%氧气+甲基牛扁亭组。观察7天动物存活率，注射酵母多糖后24小时取心、肝、肺、肾，做组织病理学以及病理学评分，测定15小时血气和24小时血浆中生化指标及炎症因子。结果：100%氧吸入可提高脓毒症动物的存活率，改善其肝肾生化指标，降低病理评分，减低炎症因子T N F水平。给予α7nAchR受体拮抗剂甲基牛扁亭后，脓毒症动物第7天存活率由80%下降到40%，各器官病理评分显著升高，测定血浆中各项生化指标显著增高，血浆中肿瘤坏死因子-α水平显著增高，且与脓毒症+100%氧气组比较，其差异均有显著统计学意义(p<0.05)。结论：100%氧对酵母多糖所致脓毒症小鼠有保护作用。α7nAchR受体拮抗剂甲基牛扁亭可逆转100%氧气对酵母多糖所致脓毒症小鼠的保护作用，表明胆碱能抗炎通路参与100%氧对酵母多糖所致脓毒症小鼠的保护效应。
Objective: Studies have shown that 100% oxygen inhalation has a protective effect on murine sepsis model, without causing oxidative stress and organ damage, suggesting that high oxygen may become an important treatment for sepsis. This article was scheduled to observe the effects of subcutaneously injected α7 nicotinic acetylcholine receptor (α7nAchR) antagonist methyl ycaconitine on protective action against zymosan-induced sepsis by 100% oxygen in mice. Methods: Animals having intraperitoneal injection of zymosan or the same volume of normal saline, were randomly divided into five groups: (1) normal saline + air; (2) zymosan + air; (3) zymosan + air + methyl ycaconitine; (4) zymosan +100% oxygen; (5) zymosan+100% oxygen + methyl ycaconitine groups. 7-day animal survival rate, heart, liver, lung, kidney tissue pathology and pathological scores as wel as plasma biochemical indices and inflammatory factors at 24 hours after zymosan injection were determined. Results: 100% oxygen inhalation improved the survival rate of animals with sepsis, improved liver and kidney biochemical indicators, and reduced the pathologic score and the level of inflammatory cytokine TNF. Given α7nAchR receptor antagonist methyl ycaconitine, the7-day animal survival rate decreased from 80% to 40%, organ pathology score was significantly increased, measured plasma biochemical indexes and plasma tumor necrosis factor-α levels were significantly increased, when compared with those in the zymosan +100% oxygen group, with statistical y significant differences (P <0.05). Conclusion: 100% oxygen has a protective effect on zymosan induced septic mice which was reversed byα7nAchR antagonist, indicating that the cholinergic anti-inflammatory pathway is involved in the protective effect of 100% oxygen on zymosan-induced sepsis.
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