Objective: To investigate the Dalian Friendship Hospital of Acinetobacter baumanni distribution characteristics of Bacilus in the clinical departments and drug resistance situation. Method: analysis of 151 strains of Acinetobacter baumanni specimen source, distribution and drug resistance of clinical departments. Results: Acinetobacter baumanni mainly isolated from sputum specimens, accounting for 40.4%; the main clinical distribution in the ICU, surgical ICU and neuro ICU; Acinetobacter baumanni resistance was serious. Conclusion: the drug resistance of Acinetobacter baumanni to various antibiotics were higher, the clinical reasonable use of antibiotics and to strengthen the monitoring of drug resistance, prevent the formation and spread of drug resistant strains.