objective to evaluate the measurement uncertainty in the clinical application of biochemical test results. methods Our hospital 86 cases of clinical patients, biochemical test extraction of venous blood, respectively, for the biochemical test, uncertainty of different measurements. Results statistical biochemical test results of 86 cases were found, gamma glutamine transferase uncertainty is 13.89, the uncertainty of Na+ 1.33, K+ 2.41, the biochemical test indexes of uncertainty exists obvious difference. Conclusion application of uncertainty for clinical biochemical test, dispersion and the accuracy of test results may be ful y reflected, effectively reduce the detection error, is worth promoting.