To analyze the effect of the application of gamma globulin in the treatment of pediatric diseases. Methods: randomly selected homes in the August 2011 to August 2012 during the application of gamma globulin treatment 78 cases of pediatric diseases, treatment of this retrospective analysis of 78 patients, and further analysis of gamma globulin in the treatment of pediatric diseases application value. Results: Patients with gamma globulin neonatal hemolytic jaundice disease condition improved after treatment, the rate is 83.3%, improvement rate of infectious diseases such as chickenpox and measles patients was 90.9%, the improvement rate of neonatal hypoxic-ischemic encephalopathy in patients was 81.2%, improvement rate was 99.9% in patients with Kawasaki disease, improvement rate in patients with severe pneumonia was 85.7%. Conclusion: The gamma globulin can enhance immunity, improve pediatric disease hemolytic jaundice, chicken pox and measles and other infectious diseases, neonatal hypoxic-ischemic encephalopathy, Kawasaki disease, severe il ness such as pneumonia, of these diseases and has better therapeutic effect.