Objective To investigate the relationship between pediatric rotavirus enteritis and serum transaminases and myocardial enzyme changes. Methods 55 cases with pediatric rotavirus enteritis and 55 cases rotavirus enteritis with with rotavirus test negative were studied. Compare and analyze serum transaminases and myocardial enzymes in the observation group and control group. Results Each monitoring indicators of serum transaminases and cardiac enzymes in patients in the observation group were significantly higher in the control group, the difference was statistical y significant (P <0.05). Conclusion Pediatric rotavirus enteritis can induce abnormal serum myocardial enzymes and transaminases; monitoring the changes of serum transaminases and myocardial enzymes early has important referential meaning for diagnosis, intervention, and treatment of pediatric rotavirus enteritis.