Objective: To compare the analysis of micro-gel method and polybrene method crossmatch test application results. Methods: Hospital from January 2013 to January 2014 recipients treated 1,278 cases for the study, and these patients were treated with micro-gel method and technology joint polybrene crossmatch test, comparing the two cross blood test results with application in practical work. Results: The micro-gel method with sub-six cases of blood, with blood substandard polybrene method has five cases, two methods were substandard four cases with blood, there is no significant difference between the two groups (P>0.05). Conclusion: The micro gel method and polybrene method has its own advantages and disadvantages of each, should be used in conjunction with blood work in order to help improve the safety of blood transfusions, more suitable for clinical use.