Objective: To investigate the distribution and drug resistance ofbacteria of respiratory tract infection, and provide the basis for clinical rational drug use. Method: to lead to patients with respiratory infection bacteria culture and sensitivity test, determination of the clinical antibiotic resistance. Results: thecultured from specimens of 568 pathogen detection, the positive rate was 34.4%.The common bacteria in respiratory tract infection: 237 strains of Pseudomonas aeruginosa, Klebsiel a pneumoniae 98 strains, 51 strains of Escherichia coli, 34 strains of Staphylococcus aureus, accounting for 73.9% of the total. Resistance to other antimicrobial agents have different levels of. Conclusion: the mainpathogenic bacteria of respiratory tract infection are gram negative bacil us infection, multiple bacterial drug resistance is serious; antibiotics should be selected according to drug sensitivity results of bacteria infection, bacteriologicalexamination is the rational use of antimicrobial drugs and the best way to reducebacterial resistance.