摘要
目的：分析结直肠癌组织中m i R-21的表达及其临床病理学意义。方法：选取我院2012年1月-2012年12月间手术切除的结直肠癌标本30例作为研究对象，通过采用TaqMan实时定量RT-PCR法对该组标本中的结直肠癌组织miR-21的表达进行检测，并与其癌旁正常组织miR-21的表达进行对比观察，从而探讨结直肠癌临床病理因素与miR-21的表达之间的关系。结果：通过与对比癌旁正常组织miR-21的表达进行对比观察发现，结直肠癌组织miR-21的表达明显要高很多，差异具有统计学意义（P<0．05）。结直肠癌组织miR-21的表达与患者的年龄、性别、肿瘤部位等情况没有较大的关联，而与其淋巴结转移、肿瘤病理学分级、及术后生存率三个方面有着紧密的关系。结论：结直肠癌组织miR-21的表达要高于正常组织，并且它可能与大肠癌的发生及肿瘤的生长有着密切的联系。
Abstract
objective: to analysis of miR - 21 expression in colorectal cancer tissues and its clinical pathological significance. Methods: from January 2012 to December 2012 surgical resection of colorectal cancer specimens from 30 cases as the research object, by adopting the TaqMan real-time quantitative rt-pcr method to this group of specimens of colorectal cancer tissue miR - 21 expression, and normal tissue adjacent to carcinoma with miR - comparing the expression of 21 observation, which discuss clinicopathological factors of colorectal cancer with the relationship between the expression of miR - 21. Results: by contrast with the normal tissue adjacent to carcinoma miR - comparing the expression of 21 observation found that miR - 21 expression of colorectal cancer tissues has much higher, the difference is statisticaly significant (P < 0.05). The expression of colorectal cancer tissue miR - 21 with the patient's age, gender, tumor location, and so on and so forth no larger correlation, and lymph node metastasis, tumor pathology classification, and postoperative survival rate three aspects have close relation. Conclusion: the expression of colorectal cancer tissue miR - 21 than in normal tissue, and it may be related to the occurrence of colorectal cancer and tumor growth is closely related.
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