Method to col ect data in December 2011 to December 2013, 2500 copies of al types of clinical microbiological abstract: objective to understand the clinical specimens at different times and different types of microbial test results positive rate distribution of specimen, the two periods were retrospectively analyzed in different kinds of clinical microbiological test results were positive. Results the rest of the specimens of al kinds of the second stage test were significantly lower than the first stage (P < 0.05). Conclusion to improve the clinical disease treatment success, we must to ensure the quality of microbial test specimens, the specimens of different time periods and different types of test positive rate distribution for detailed analysis.