标本的不同保存条件对实时荧光定量HCV-RNA检测的影响
Impact of the different preservation for the detection of serum HCV RNA using FQ-RT-PCR
韩亚男 1许琳婧 1王迪1
作者信息
- 1. 辽宁大连市第六人民医院中心实验室 116031
- 折叠
摘要
目的：了解标本保存对实时荧光定量HCV-RNA检测结果的影响。方法收集20份丙肝患者的血标本分离血清后，把每份血清标本分成6份分别放置在室温、4℃和-20℃条件下保存1天和1周后用荧光定量PCR法检测结果，采用SPSS 17.0软件对数据进行处理与相关分析。结果血清分别在室温、4℃和-20℃条件下保存1天和1周对HCV-RNA的荧光定量检测结果无统计学差异。结论在保存1周时间内，血清HCV-RNA的荧光定量检测结果不受标本保存温度和保存时间的影响。
Abstract
Objective To investigate the impact of various storage conditions of serum samples on HCV RNA detection.Methods Twenty examples of hepatitis C patients were preserved at six different condition and detected by Real-time fluorenscent quantitative PCR. Results The results of HCV RNA in serum samples which were preserved 1 day and 1 week at room temperature,4℃and -20℃ showed no significant difference. Conclusion Preserved temperature and preserved time have no effect on test results.
关键词
HCV-RNA/FQ-RT-PCR/标本保存Key words
The preservation of the specimen/Hepatitis C virus RNA/Fluorescence quantitative PCR引用本文复制引用
出版年
2014
NETL
NSTL
万方数据