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标本的不同保存条件对实时荧光定量HCV-RNA检测的影响

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目的:了解标本保存对实时荧光定量HCV-RNA检测结果的影响。方法收集20份丙肝患者的血标本分离血清后,把每份血清标本分成6份分别放置在室温、4℃和-20℃条件下保存1天和1周后用荧光定量PCR法检测结果,采用SPSS 17.0软件对数据进行处理与相关分析。结果血清分别在室温、4℃和-20℃条件下保存1天和1周对HCV-RNA的荧光定量检测结果无统计学差异。结论在保存1周时间内,血清HCV-RNA的荧光定量检测结果不受标本保存温度和保存时间的影响。
Impact of the different preservation for the detection of serum HCV RNA using FQ-RT-PCR
Objective To investigate the impact of various storage conditions of serum samples on HCV RNA detection.Methods Twenty examples of hepatitis C patients were preserved at six different condition and detected by Real-time fluorenscent quantitative PCR. Results The results of HCV RNA in serum samples which were preserved 1 day and 1 week at room temperature,4℃and -20℃ showed no significant difference. Conclusion Preserved temperature and preserved time have no effect on test results.

The preservation of the specimenHepatitis C virus RNAFluorescence quantitative PCR

韩亚男、许琳婧、王迪

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辽宁大连市第六人民医院中心实验室 116031

HCV-RNA FQ-RT-PCR 标本保存

2014

中外健康文摘
中国中医药报社

中外健康文摘

影响因子:0.016
ISSN:1672-5085
年,卷(期):2014.(14)
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