Objective: the application effects of “The Checking Sheets of Operation Safety” is discussed in the essay. Methods: operation safety verification system and process are executed strictly. The identity and surgical site of the patients are verified by anesthetists, surgery doctor and nurses separately in the process of anesthesia, before and after the operation. Achievements: The work of surgery is accomplished better than before, and security of surgical patient is highly improved. Medical dispute and surgery mistake are eliminated. Conclusion: the implements of “The Checking Sheets of Operation Safety” can ensure the safety of surgery, decrease the mistake and raise the quality of service.