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浅谈幽门螺杆菌临床检验方法

Discussion on Helicobacter pylori clinical test method

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目的:用不同方法检测幽门螺杆菌,评价最有效的方法,为临床检验提供有利依据。方法统计分析了400例感染有幽门螺杆菌的患者临床资料。分离胃活检标本中的幽门螺杆菌,并用尿素酶试验、涂片镜检方法以及幽门螺杆菌抗体ELISA检测的方法进行对比鉴定研究。结果嗜银染色法检验阳性者181例(45.3%),快速尿素酶法检验阳性者76例(19.0%),两种方法检验均显阳性者135例(33.75%);嗜银染色法检验阳性、快速尿素酶法检验阴性6例(1.5%),嗜银染色法检验阴性性、快速尿素酶法检验阳性36例(9.0%)。结论嗜银染色法特异性比较高,血清H p 抗体对测定非侵入性幽门螺杆菌,准确率较高,且对患者不会造成较大损伤。在临床上可采用联合诊断法来提高幽门螺杆菌的诊断准确性。
objective to use different methods for detecting Helicobacter pylori, the most effective method of evaluation, which provides basis for the clinical laboratory. Methods statistical analysis of 400 cases clinical data of patients infected with Helicobacter pylori. Helicobacter pylori in gastric biopsy specimens of comparative research, and identified by urease test, smear method and method of Helicobacter pylori ELISA antibody detection. Results 181 cases of Argyrophil staining test positive (45.3%), 76 cases of rapid urease test positive (19%), two kinds of methods were significantly positive in 135 cases (33.75%); argyrophilic staining test positive, rapid urease test was negative in 6 patients (1.5%), argyrophil staining test was negative. Sex, rapid urease test was positive in 36 patients (9%). Conclusion the argyrophil staining specificity is high, serum Hp antibody on the determination of non-invasive Helicobacter pylori, higher accuracy, and caused great damage to the patients don't. In clinical on can be used to improve the diagnostic accuracy of combined diagnosis of Helicobacter pylori.

Helicobacter pylorisilver stainingELISA detectionClinical Laboratory

郭德珊

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四川省阿坝藏族羌族自治州人民医院检验科 624000

幽门螺杆菌 嗜银染色法 ELISA检测 临床检验

2014

中外健康文摘
中国中医药报社

中外健康文摘

影响因子:0.016
ISSN:1672-5085
年,卷(期):2014.(17)
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