Objective: To study the distribution and drug resistance of Acinetobacter Baumanni by clinical sample isolation and culture. Methods: Colected clinical samples in this hospital from March 2012 to March 2014 and took the identification of bacteria and drug sensitivity test by VITEK2 automatic bacteria assay drug sensitive analyzer. Results: Among 126 strains of Acinetobacter Baumanni, most of them (76.2%) were isolated from sputu m sample, while the distribution in intensive care unit occupied the major part (40.5%). For clinical common drugs, Acinetobacter Baumanni had a good sensitivity to minocy cline and cefoperazone/sulbactam (>90%), but a poor sensitivity to other clinical common drugs (<50%). Conclusion: The multiple drug resistance of Acinetobacter Baumann-i is severe and it should enhance the supervision on drug resistance to reduce and avoid the in-hospital infection induced by Acinetobacter Baumanni.