Objective To understand the management and maintenance on the quality of the clean operating room operation, explore scientific and effective management mode. Method Using the FSC - Ⅴ plankton sampler colecting operating room air, 37℃ cultivate 24 h count. In the operating room management, equipment maintenance situation by questionnaire survey and field checking method. Results Management of a hospital static monitoring planktonic bacteria colony count an average of 65/m3, the dynamic monitoring of planktonic bacteria colony count an average of 160/m3;Management good b average hospital static monitoring planktonic bacteria colony number 27/m3, the dynamic monitoring of planktonic bacteria colony count on average 30/m3. Conclusion To give ful play to clean equipment efficiency, improve equipment efficiency, creating high quality clean operation environment, must establish a set of scientific and effective management and equipment maintenance mechanism is the key to ensure the quality of clean operating room to run.