Objective To determine the content of Berberine hydrochloride in ruyijinhuang pils Methods Using TLC-scanning to determinate the content of berberine hydrochloride.Results TLC-scanning can remove the interference of other alklorids,the average recovery is 101.2%,RSD=1.05%,this method is simple ,accurate and with a good reproducibility .Conclusion This method can determinate the content of ruyijinhuang pils.