to study and analysis of pharmaceutical interventions on the use of quinolones and the effect of resistance. Methods: randomly choose a hospital for the first half of this year and last year the first half of prescription drugs. Watch the first half of this year the pharmaceutical intervention for the use of quinolones in case for the observation group and the first half of last year did not use any medicine intervention is the use of quinolones notes for the control group, and compared to observe the situation of the two groups of drug dosage, drug resistance and is not required to the incidence of drug use, etc. Results: the observation and comparison, the control group in the resistance to disease, preoperative prevention using dosage, drug produced strong resistance and a high incidence of irrational drug use and the doctor on drug use situation awareness lower, there are differences between the two groups. Conclusion: the use of quinolones in pharmaceutical intervention, under the condition of reasonable utilization rate increases, drug resistance wil reduce, used in the clinical treatment is effective and feasible.