To investigate the humane care in the operating room nursing application and effect. Methods: 102 cases treated my department to analyze the operating room patients were randomly divided into observation group and control group, two groups of patients after effective treatment, the observation group were implemented humane care, routine care measures implemented in the control group of patients, two group satisfaction and quality of patient care were compared. Results: The implementation of human observation group patient care initiatives, the satisfaction and the quality of care was significantly better than the control group, a significant difference (P <0.05), with a clinical comparability. Conclusion: constantly improve operating room nursing work system, the implementation of humane care measures to improve customer satisfaction and quality of care patients have a very important role.