Objective By using the method of correlation analysis and discussion of the clinical aspects of multi pathogen infection of patients in pediatric myocarditis;Method Our hospital adopts methods ELISA medical detection of virus infection in children with myocarditis in vivo source to participate in the study.Result After a thorough inspection, a total of 46 cases were obtained, which belongs to the Coxsackie B3 virus (CVB3) and positive with a total of 27 cases, the total probability 58.69%; Ai Ke (ECHO) virus and positive with a total of 9 cases, the total probability of mycoplasma pneumonia (MP belongs to 19.56%;) and positive with a total of 4 cases, the total probability 8.69%; belongs to EP virus and positive with a total of 3 cases, the total probability 6.52%; another 3 cases using ELISA method did not detect pathogens in children, accounting for a total probability of 6.52%;Conclusion Clinical pediatric myocarditis is a very common cardiovascular disease in children, but the disease stil exist in a variety of virus infection in patients with joint may, in clinical treatment should be treated more added to the different infection pathogen.