Microfluidic chip detection technology and its application prospect in plant quarantine
Microfluidic chip technology is an advanced biotechnology that enables precise manipulation of droplets at the nano-scale and micro-scale within narrow channels,facilitating high-throughput detection.This paper provided a concise overview of the advancements and advantages associated with microfluidic chip materials and processing techniques.It primarily focused on integrating this technology with recom-binase polymerase amplification(RPA),loop-mediated isothermal amplification(LAMP),quantitative real-time polymerase chain reaction(qPCR),and others,along with its principles and application cases in high-through-put detection of microorganisms and genetically modified organisms.The potential applications for the de-tection of hazardous pests in plant quarantine were also discussed.Comprehensive analysis showed that microfluidic chip technology has several advantages,including minimal reagent consumption,rapid detec-tion speed,high throughput,and user-friendly operation.Its broad development space and application prospects lie in its capacity to facilitate efficient high-throughput detection of specific hazardous pests or all hazardous pests on specific plants or plant products.