Establishment of KASP identification method for Amaranthus tuberculatus
The identification of Amaranthus tuberculatus from other species in the same genus has al-ways been a challenge.This study established a KASP identification method for the A.tuberculatus.It was used for rapid and accurate molecular identification.23 samples of 11 species of A.tuberculatus were selected as materials.Based on the Amaranthus palmeri nuclear genome sequence,the primers AP4F and AP4R were designed.A Single nucleotide polymorphism(SNP)site was found through sequence am-plification and comparison.The KASP reaction primers for identification of A.tuberculatus were designed using Primer design software Primer 5.The results showed that the specific primers amplified by KASP technology could form obvious genotyping,which could distinguish A.tuberculatus from related species of the same genus.This study provided a new technique for the detection and identification of A.tubercula-tus in imported grain.
NETL
NSTL
万方数据
维普
