Establishment of real-time PCR detection method for Pantoea stewartii subsp.stewartii
Pantoea stewartii subsp.stewartii(Pss)is one of import plant quarantine pests in china.The specific-Pss primer and probe were designed by comparing the sequence difference between Pss and the related species,and established a real-time fluorescence PCR detection method.The results showed that only 6 strains of Pss had positive amplification,other 74 strains and blank control were negative.The low limit of detection was 13 fg of DNA.The established real-time fluorescence PCR method was used to de-tect the simulated infected maize seeds and the standard curve was established.It was found that R2=0.998 8,showing a good linear relationship.The specific and sensitive PCR method can provide a more accurate and rapid detection method for port quarantine.
万方数据
维普
