Development of RPA assay for detection of the corn rust pathogens Puccinia polysora Underw.and Puccinia sorghi Schw
Corn rust is one of the destructive diseases in global corn production,and the spores of the pathogen can spread with the airflow,easily causing a pandemic of the disease and causing significant losses to China's corn production.This study designed primers based on the ITS sequences of Puccinia polysora Underw.and Puccinia sorghi Schw.,and established a detection method based on Recombinase Polymerase Amplification(RPA).The sensitivity of the RPA detection method for Puccinia polysora Underw.can reach 7.85 pg/p,L.The sensitivity of the RPA detection method for Puccinia sorghi Schw.can reach 9.9 pg/μL.After specific validation,two different strains of corn rust can be effectively distinguished,and field samples have been validated.The results show that the RPA specific primers designed in this experiment have good detection effects and are easy to operate.It provides a new method for frontline personnel to quickly identify Puccinia polysora Underw.and Puccinia sorghi Schw.,which is of great significance for early warning and prevention of corn rust in China.
万方数据
维普
