摘要
从荷兰进境风信子种球软腐症状组织处分离到一株分离物2018-5,对分离物进行PCR检测、荧光PCR检测、16S rRNA序列分析、多位点序列分析和致病性测试.分离物在NA平板上菌落淡黄褐色,扁平,圆形,边缘不规则.测试结果表明,常规PCR和荧光PCR检测均为阳性;16S rRNA、rpoS、rplB、recA、purA、gapA、gyrA、fusA、dnaX序列和达旦提狄克氏菌达旦提亚种(Dickeya dadantii subsp.dadantii)菌株NCPPB898(CP023467)的序列相似性分别为99.93％,99.55％,100％,95.68％,99.35％,99.34％,99.37％,99.86％,99.59％;基于8个看家基因(rpoS、rplB、recA、purA、gapA、gyrA、fusA和dnaX)的系统发育树显示,分离物2108-5与Dickeya dadantii subsp.dadantii处于同一分支;人工接种风信子叶片和甘薯幼茎都能引起腐烂反应.根据试验结果将分离物2018-5鉴定为达旦提狄克氏菌达旦提亚种(Dickeya dadantii subsp.dadantii).
Abstract
A strain 2018-5 isolated in hyacinth bulbs with soft rot symptom imported from the Nether-lands was identified by PCR detection,real time PCR detection,16S rRNA sequence analysis,MLST(Mul-tilocus Sequence Typing),pathogenicity test.The colony of the isolate on the NA plate is yellowish brown,flat,round and irregular.The results showed that both conventional PCR and real time PCR were positive of Dickeya dadantii subsp.dadantii;The sequence similarity of 16S rRNA,rpoS,rplB,recA,purA,gapA,gyrA,fusA,dnaX and Dickeya dadantii subsp.dadantii strain NCPPB898(CP023467)were 99.93％,99.55％,100％,95.68％,99.35％,99.34％,99.37％,99.86％,99.59％,respectively;The phylogenetic tree based on 8 housekeeping genes(rpoS,rplB,recA,purA,gapA,gyrA,fusA and dnaX)showed that the isolate 2108-5 was in the same branch as D.dadantii subsp.dadantii;Artificial inoculation of hyacinth leaves and sweet potato young stems can cause rot reaction.According to the test results,the isolate 2018-5 was identified as Dickeya dadantii subsp.dadantii.
基金项目
上海市科技兴农项目(2021-02-08-00-12-F00771)
NETL
NSTL
万方数据