Isolation and identification of Dickeya dadantii subsp.dadantii in hyacinth bulbs imported from the Netherlands
A strain 2018-5 isolated in hyacinth bulbs with soft rot symptom imported from the Nether-lands was identified by PCR detection,real time PCR detection,16S rRNA sequence analysis,MLST(Mul-tilocus Sequence Typing),pathogenicity test.The colony of the isolate on the NA plate is yellowish brown,flat,round and irregular.The results showed that both conventional PCR and real time PCR were positive of Dickeya dadantii subsp.dadantii;The sequence similarity of 16S rRNA,rpoS,rplB,recA,purA,gapA,gyrA,fusA,dnaX and Dickeya dadantii subsp.dadantii strain NCPPB898(CP023467)were 99.93％,99.55％,100％,95.68％,99.35％,99.34％,99.37％,99.86％,99.59％,respectively;The phylogenetic tree based on 8 housekeeping genes(rpoS,rplB,recA,purA,gapA,gyrA,fusA and dnaX)showed that the isolate 2108-5 was in the same branch as D.dadantii subsp.dadantii;Artificial inoculation of hyacinth leaves and sweet potato young stems can cause rot reaction.According to the test results,the isolate 2018-5 was identified as Dickeya dadantii subsp.dadantii.
NETL
NSTL
万方数据
