基于RPA的菲利普木蠹象分子鉴定技术
Molecular identification of Pissodes piniphilus(Herbst)based on RPA
刘玮琦 1李兰 2梁靓 3刘振伟 1赵畅 1陈超 1丛日赫 1王旭1
作者信息
- 1. 满洲里海关技术中心内蒙古满洲里 021400
- 2. 阿拉山口海关技术中心
- 3. 西安海关技术中心
- 折叠
摘要
为建立快速、灵敏检测菲利普木蠹象的重组酶聚合酶扩增检测(RPA)方法,本研究利用菲利普木蠹象的COI基因序列,设计筛选特异性引物PH3,并对引物特异性和灵敏度进行检测.结果表明,设计的引物能够特异检测出菲利普木蠹象,该引物检测菲利普木蠹象的灵敏度为0.045 ng/μL.所建立的RPA方法特异性强、灵敏度高,可用于口岸一线对菲利普木蠹象的检测.
Abstract
To establish a rapid and sensitive recombinase polymerase amplification assay(RPA)method for the detection of Pissodes piniphilus.This study designed and screened specific primers PH3 based on the COI gene sequence of Pissodes piniphilus,and detected the specificity and sensitivity of the primers.The results showed that the designed primer was able to specifically detect Pissodes piniphilus,with a sensitivity of 0.045 ng/μL.The established recombinase polymerase amplification assay(RPA)method has strong specificity and high sensitivity for detecting Pissodes piniphilus at port.
关键词
菲利普木蠹象/RPA技术/分子鉴定Key words
Pissodes piniphilus/RPA technology/molecular identification引用本文复制引用
基金项目
海关总署科研项目(2020HK1742022HK107)
出版年
2024
NETL
NSTL
万方数据