Molecular identification of Pissodes piniphilus(Herbst)based on RPA
To establish a rapid and sensitive recombinase polymerase amplification assay(RPA)method for the detection of Pissodes piniphilus.This study designed and screened specific primers PH3 based on the COI gene sequence of Pissodes piniphilus,and detected the specificity and sensitivity of the primers.The results showed that the designed primer was able to specifically detect Pissodes piniphilus,with a sensitivity of 0.045 ng/μL.The established recombinase polymerase amplification assay(RPA)method has strong specificity and high sensitivity for detecting Pissodes piniphilus at port.
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