Identification and bioinformatics analysis of FoccIR01 on Frankliniella occidentalis
In order to explore the function and mechanism of IRs and their application in the prevention and control of Frankliniella occidentalis,sequence analysis was performed using DNAMAN software,ho-mology comparison was conducted with BLAST,and a phylogenetic tree was constructed using MEGA 6's neighbor-joining method.Its sequence characteristics and phylogenetic status were studied in this paper.An ionic receptors gene named FoccIR01(GenBank accession number:PP314350)was successfully cloned in Frankliniella occidentalis.The full length of FoccIR01 cDNA is 3 103 bp,contains a 2 415 bp open reading frame(ORF)encoding a putative protein of 805 amino acids with a molecular mass of 90010.90 Da and an isoelectric point of 6.95.The non-coding regions of the 3'end and 5'end are 217 bp and 468 bp,respectively.The results from the homology similarity search showed that insects belonging to the order Lepidoptera were the most,accounting for 60％of the total.Evolutionary system analysis showed that FoccIR01 has the closest phylogenetic relationship with FoccIR40aX3(GenBank accession number:XP_052122665.1),FoccIR40a(GenBank accession number:KAE8747820.1),FoccIR40aX1(GenBank accession number:XP_052122663.1)and FoccIR40aX2(GenBank accession number:XP_052122664.1)from Franklin-iella occidentalis,followed by TpalIR40a(GenBank accession number:XP_034250854.1)in Thrips palmi,MusiHP(GenBank accession number:KAJ1529788.1)in Megalurothrips usitatus and IeleIR40a(GenBank accession number:XP_046401562.1)in Ischnura elegans,which indicated that FoccIR01 is relatively closely related to them.Clarified the nucleotide and amino acid sequence characteristics of FoccIR01,laid the foundation for further research on the specific role of this protein gene in the behavioral activities of Frankliniella occidentalis.
万方数据
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