摘要
为建立窄穗组常见入侵杂草的DNA条形码鉴定方法,选取核基因组的ITS2、ITS,叶绿体基因组的matK、ndhF、trnT-trnL、rpl32-trnL、rpoB-trnC和trnC-petN等8个片段进行筛选.综合进化树、种间差异,barcoding gap等多项指标,结果表明trnT-trnL、rpl32-trnL、trnC-petN和rpoB-trnC序列鉴定效果相对较好,ITS2、ndhF、ITS和matK序列分辨能力较差.基于叶绿体基因组的trnT-trnL和rpl32-trnL序列,在扩增测序的成功率、种间变异、物种鉴定能力、聚类分析等均表现较好,虽然在一些种内存在较高的种内变异,在形态鉴定的基础上可作为窄穗组DNA条形码鉴定的推荐片段.
Abstract
We tested the applicability of eight DNA regions(ITS2、ITS、matK,ndhF,trnT-trnL,rpl32-trnL,rpoB-trnC and tmC-petN)as the barcodes for identifying invasive weeds in Bromus Sect.Genea.The re-sults showed that trnT-trnL,rpl32-trnL,trnC-petN and rpoB-trnC sequences were better identified,as ITS2,ndhF,ITS and matK sequences were poorly resolved.The chloroplast genome-based trnT-trnL and rpl32-trnL sequences performed well in terms of amplification and sequencing success,interspecific varia-tion,species identification ability,and cluster analysis,despite high intraspecific variation in some species.Based on morphological identification,these sequences can be recommended as DNA barcode fragments for Bromus Sect.Genea.
维普
万方数据