Screening of DNA barcodes for weeds of Bromus Sect.Genea
We tested the applicability of eight DNA regions(ITS2、ITS、matK,ndhF,trnT-trnL,rpl32-trnL,rpoB-trnC and tmC-petN)as the barcodes for identifying invasive weeds in Bromus Sect.Genea.The re-sults showed that trnT-trnL,rpl32-trnL,trnC-petN and rpoB-trnC sequences were better identified,as ITS2,ndhF,ITS and matK sequences were poorly resolved.The chloroplast genome-based trnT-trnL and rpl32-trnL sequences performed well in terms of amplification and sequencing success,interspecific varia-tion,species identification ability,and cluster analysis,despite high intraspecific variation in some species.Based on morphological identification,these sequences can be recommended as DNA barcode fragments for Bromus Sect.Genea.
万方数据
维普
