Detection of Seifertia azaleae by PCR and real-time PCR
The primer SA1/SA2 and probe SA-P based on the ITS sequence of S.azalea and related species in GenBank was developed.A PCR and real-time PCR method for the detection of Seifertia aza-leae were established.The continental PCR with primer SA1/SA2 and the real time PCR with probe SA-P could got the positive result for 5 strains of S.azaleae,but no positive amplification was obtained for other strains.The detection sensitivity of continental PCR can reach 60 pg of mycelium DNA,Probe SA-P can reach 60 fg of mycelium DNA,which is 1 000 times higher than conventional PCR.The detection assay for the flower bud and leaf samples of rhododendron showed potential role for rapid detection of S.azaleae in imported rhododendron seedlings.
万方数据
维普
