The primer SA1/SA2 and probe SA-P based on the ITS sequence of S.azalea and related species in GenBank was developed.A PCR and real-time PCR method for the detection of Seifertia aza-leae were established.The continental PCR with primer SA1/SA2 and the real time PCR with probe SA-P could got the positive result for 5 strains of S.azaleae,but no positive amplification was obtained for other strains.The detection sensitivity of continental PCR can reach 60 pg of mycelium DNA,Probe SA-P can reach 60 fg of mycelium DNA,which is 1 000 times higher than conventional PCR.The detection assay for the flower bud and leaf samples of rhododendron showed potential role for rapid detection of S.azaleae in imported rhododendron seedlings.
维普
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