Root knot nematodes are specific endoparasitic nematodes that can invade the interior of host plant roots and causing damage.They are one of the most economically important nematode groups.The common form is the second juvenile stage,which is very small,showing similar interspecies morphology and significant intraspecies differences.Adults are difficult to obtain,males typically lack distinctive species identification characteristics,while the perineal patterns of females are highly similar,making mor-phological identification challenging.Building upon Subbotin et al.'s published polytomous keys,this arti-cle further organizes the table to provide a reference for domestic agricultural or customs ports inspection in root knot nematode quarantine and identification.