Detection of three quarantine viruses in imported sorghum.
This study used imported sorghum as material to detect maize chlorotic mottle virus(MCMV),maize dwarf mosaic virus(MDMV),and wheat stripe mosaic virus(WSMV)based on one-step RT-PCR.Among 23 sorghum samples,2 samples were positive for MCMV,5 samples were positive for MDMV,and 19 samples were positive for WSMV.One sample tested positive for MCMV,MDMV and WSMV,while 4 samples were positive for MDMV and WSMV.It is recommended to use MCMV-F7/MCMV-R7,MDMV482F/MDMV482R and WSMV-F3/WSMV-R3 as the preferred primers for the detection of three quarantine viruses.MCMV-2452F/MCMV-3111R,3MDF/1MDR and WSMV-1F/WSMV-3R are considered as candidates,and the primer 3MDF/1MDR is used to identify the phylogroup of MDMV isolates.
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