进口高粱中3种检疫性病毒的检测
Detection of three quarantine viruses in imported sorghum.
陈红运 1廖富荣 1陈青 1叶明辉 1冯黎霞 2于翠 3王秀芬4
作者信息
- 1. 厦门海关技术中心 福建厦门 361026
- 2. 广州海关技术中心
- 3. 上海海关动植物与食品检验检疫技术中心
- 4. 大连海关技术中心
- 折叠
摘要
本研究以进口高粱为材料,采用一步法RT-PCR检测玉米褪绿斑驳病毒(maize chlorotic mottle virus,MCMV)、玉米矮花叶病毒(maize dwarf mosaic virus,MDMV)和小麦线条花叶病毒(wheat streak mosaic virus,WSMV).23份高粱样品中,2份检出MCMV,5份检出MDMV,19份样品检出WSMV.1份样品检出MCMV、MDMV和WSMV,4份样品检出MDMV和WSMV.综合前期和本研究结果,建议将MCMV-F7/MCMV-R7、MDMV482F/MDMV482R 和 WSMV-F3/WSMV-R3 作为 3 种检疫性病毒的首选检测引物,MCMV-2452F/MCMV-3111R、3MDF/1MDR 和 WSMV-1F/WSMV-3R 作为备选,引物 3MDF/1MDR 用于鉴定MDMV分离物的分支归属.
Abstract
This study used imported sorghum as material to detect maize chlorotic mottle virus(MCMV),maize dwarf mosaic virus(MDMV),and wheat stripe mosaic virus(WSMV)based on one-step RT-PCR.Among 23 sorghum samples,2 samples were positive for MCMV,5 samples were positive for MDMV,and 19 samples were positive for WSMV.One sample tested positive for MCMV,MDMV and WSMV,while 4 samples were positive for MDMV and WSMV.It is recommended to use MCMV-F7/MCMV-R7,MDMV482F/MDMV482R and WSMV-F3/WSMV-R3 as the preferred primers for the detection of three quarantine viruses.MCMV-2452F/MCMV-3111R,3MDF/1MDR and WSMV-1F/WSMV-3R are considered as candidates,and the primer 3MDF/1MDR is used to identify the phylogroup of MDMV isolates.
关键词
玉米褪绿斑驳病毒/玉米矮花叶病毒/小麦线条花叶病毒/检测Key words
maize chlorotic mottle virus/maize dwarf mosaic virus/wheat streak mosaic virus/detection引用本文复制引用
基金项目
海关总署科研项目(2022HK009)
海关总署科研项目(2024HK040)
出版年
2024
NETL
NSTL
万方数据