Development of recombinase polymerase amplification assay for detection of Anguina funesta
Anguina funesta is an invasive pest with a high risk of transmission and spread,which has ex-tremely harmful to animal husbandry production.In order to detect A.funesta accurately and effectively,specific primers were designed based on the conserved regions of the 28S rRNA-D2/D3 to develop a recom-binase polymerase amplification(RPA)assay for the detection of A.funesta.The results showed that the RPA assay proved to be specific,a fragment of 230 bp was amplified from A.funesta by specific primers.The sensitivity test showed that the RPA assay can detect 1/160 of a single nematode DNA.The results indicate the RPA assay method had advantages of rapidity,specificity,sensitivity,and suitable for rapid detection of A.funesta.
NETL
NSTL
万方数据
