Development of digital PCR detection method for Diaporthe helianthi on sunflower
In this study,the primer-probe of national standard GB/T 31792-2015"Quarantine and Identifi-cation Method of Sunflower Stem Ulcer Pathogen"was used,and the digital PCR detection technology of Diaporthe helianthi was established by constructing positive plasmid and testing its specificity,sensitivity,and repeatability.The test results showed that the primers and probes had specific amplification for 4 strains of Diaporthe helianthi,while the other 14 strains had no amplification reaction.The minimum de-tection limit of this test method is 0.008 pg/µL;The RSD of the repeatability test was less than 25％,which shows that it has good repeatability.By detecting 14 samples collected,no Diaporthe helianthi was detected.Therefore,this method has strong specificity,high sensitivity,and stable repeatability,providing re-liable and effective technical support for early monitoring and early warning of Diaporthe helianthi.
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