Development of LAMP assay for rapid detection of Ditylenchus dipsaci
Ditylenchus dipsaci is a globally recognized dangerous nematode that can cause various de-structive plant diseases and is listed as a quarantine pest in China.This study compared the sequences related to Ditylenchus in the GenBank database,and chose to use the rDNA-ITS sequence of D.dipsaci as the target.Then LAMP specific primers were designed and a visual LAMP detection system was es-tablished.The results showed that the LAMP detection method can detect D.dipsaci within 1 hour.Meanwhile,only D.dipsaci showed amplification,and the other 22 plant nematodes didn't amplify.The detection sensitivity can reach 1/4 000 scale of D.dipsaci.Furthermore,the detection results can be di-rectly judged with the naked eye.Therefore,this method has the advantages of short reaction time,high sensitivity,and low equipment requirements,making it suitable for grassroots laboratories to carry out the detection of D.dipsaci.
万方数据
维普
