Establishment and application of TaqMan probe real-time PCR method for identification of Nostoc flagelliforme
In this study,specific primer and probe were designed and selected based on partial gene se-quence encoding the Nostoc flagelliforme histidine kinase(HK).A real-time PCR method was developed for identification of Nostoc flagelliforme accordingly.The N.flagelliforme samples from different sources and closely related samples were deteted by real-time PCR.The specificity and sensitivity was determined.Specificity analysis results showed that typical amplification curves were obtained for 11N.flagelliforme samples,but not for 16 other samples such as N.paludosum,N.linckia.It indicated that the method has good specificity.The detection sensitivity was calculated to be 5.0 fg/μL in 20 μL reaction system.The established real-time PCR method in this study enables rapid,sensitive and specific identification of N.flagelliforme.It is suitable for authenticity verification of N.flagelliforme and their products.
万方数据
维普
