摘要
MYB转录因子在响应各类非生物胁迫中发挥着重要作用.本研究以'阳光玫瑰'葡萄为材料,鉴定了1个盐胁迫响应MYB转录因子基因VvMYB30,并对其特性及功能进行研究.该基因cDNA全长为984 bp,编码327个氨基酸.生物信息学分析发现,VvMYB30含有1个保守的R2R3蛋白结构域,与甜橙CsMYB30转录因子的同源性最高,相似度为64.75%,归属于SubgroupⅠ亚类.亚细胞定位及转录激活分析表明,VvMYB30定位于细胞核,且具有转录激活活性.qRT-PCR结果表明,VvMYB30受盐胁迫诱导,处理24 h表达量达到最高,为对照的6.86倍.将VvMYB30基因转化拟南芥,盐胁迫下VvMYB30转基因拟南芥种子萌发率和幼苗的成活率均显著高于野生型,表明过表达VvMYB30可增强转基因拟南芥的耐盐性.
Abstract
MYB transcription factors play an important role in responding to various abiotic stresses.This study used'Shine Muscat'grape as the material to clone a salt stress responsive MYB transcription factor gene VvMYB30,and conducted research on the characteristics and functions of VvMYB30.The sequence analysis indicated that the full-length coding sequence of VvMYB30 was 984 bp and encoded 327 amino acids.Bioinformatics analysis showed that VvMYB30 contained a conserved R2R3 domain and had the highest homology with sweet orange CsMYB30 transcription factors(64.75％similarity)and belonged to subgroup Ⅰ.Subcellular localization and transcriptional activation analysis showed that VvMYB30 was localized in the nucleus and had transcriptional activation activity.qRT-PCR showed that VvMYB30 was induced by salt stress,and the expression level reached the highest at 24 h,which was 6.86 times higher than the control.The salt tolerance test showed that the seed germination rate and seedling survival rate of VvMYB30 transgenic Arabidopsis were significantly higher than the wild type under salt stress,it indicated that overexpression of VvMYB30 could enhance the salt tolerance of transgenic Arabidopsis.
基金项目
镇江市重点研发计划(NY202002)
江苏农林职业技术学院科技项目(2020kj040)
江苏省"333高层次人才培养工程"培养对象项目(第六期)(第三层次)()
国家科技期刊平台
NSTL
维普
万方数据