首页|脱落酸调控'品丽珠'葡萄果实白藜芦醇合成的研究

脱落酸调控'品丽珠'葡萄果实白藜芦醇合成的研究

扫码查看
本研究在'品丽珠'葡萄始熟期前10 d左右进行外源脱落酸(ABA)及其合成抑制剂氟啶酮(Fluridone)处理,以去离子水处理为对照,研究外源ABA对葡萄果实白藜芦醇(Resveratrol,Res)合成与积累的调控作用。结果发现,与对照相比,外源ABA处理能够促进'品丽珠'葡萄果实提前成熟,采收时果实中可溶性固形物含量增加约5%,Res含量增加约33%;氟啶酮处理可以延缓葡萄果实成熟,采收时果实中可溶性固形物含量降低约20%,Res含量降低约44%;且葡萄果实发育过程中Res合成受转录水平的调控,外源ABA处理后ABA合成基因VvNCED1和VvNCED2表达升高,Res合成相关基因及其调控基因(VvSTS、VvMyb14及VvMyb15)表达升高,而氟啶酮处理均表现降低。进一步证明外源ABA有利于葡萄果实Res的合成和积累。
Study on Abscisic Acid Regulating Resveratrol Synthesis in'Cabernet Franc'Grape
Exogenous abscisic acid(ABA)and its synthesis inhibitor(fluridone)were applied on'Cabernet Franc'(V.vinifera L.)at about 10 days before veraison and deionized water treatment was used as the control,to investigate the transcriptional regulatory mechanism of resveratrol(Res)synthesis.From the concentration of Res and gene expression analysis,ABA advanced berry ripening,and increased the total soluble solids(5%),and Res(33%)accumulation in berries;while fluridone delayed ripening,and decreased the total soluble solids(20%)and Res(44%)accumulation.However,the synthesis of Res was regulated on transcription level during berry ripening,two ABA synthesis genes(VvNCED1 and VvNCED2)were up-regulated,as well as Res synthesis genes and their regulatory genes(VvSTS,VvMyb14 and VvMyb15)were increased in exogenous ABA-applied berries.On the other hand,these genes were down-regulated in fluridone-applied berries.Therefore,this study can further verify exogenous ABA is beneficial to the synthesis and accumulation of Res in grapes.

resveratrolabscisic acidCabernet Francgene expression

王俊芳、姜凯凯、杨东岳、孙玉霞、管雪强

展开 >

山东省葡萄研究院/山东省酿酒葡萄与葡萄酒技术创新中心,山东济南 250100

白藜芦醇 脱落酸 品丽珠 基因表达

山东省自然科学基金面上项目山东省葡萄研究院引导基金新疆生产建设兵团十二师重点领域科技攻关计划山东省科技型中小企业创新能力提升工程项目

ZR2022MC16831701887SDAIT-06-142021TSGC1239

2024

中外葡萄与葡萄酒
中国酿酒工业协会 山东省酿酒葡萄科学研究所

中外葡萄与葡萄酒

北大核心
影响因子:0.46
ISSN:1004-7360
年,卷(期):2024.(1)
  • 29