The morbidity of fruit stain on Vitis rotundifolia berries from the Nanning region of Guangxi was investigated by five-points sampling method from 2019 to 2021.Single mildew spots with different characteristics were collected and used to isolate suspected pathogenic strains.The stains were isolated and purified,then determined the pathogenicity by the Koch's postulates.Pathogenic stains with pathogenicity of fruit stain were identified by morphological and molecular methods.The results showed that the fruit stain incidence in the last three years were 92.00%,96.67%and 97.33%,with the morbidity index were 62.13,67.46 and 66.40,respectively.Multiple dominant pathogenic strains with similar morphological features were isolated from mildew spots,the representative strain GX-2216 was identified as Moesziomyces aphidis,which was closely related to the fruit stain pathogen earlier isolated from nearby'Kyoho'grape berries,indicated that M.aphidis could infect several Vitis varieties and may be the dominant fruit stain pathogen in local vineyard.
关键词
污果病/圆叶葡萄/病原菌鉴定/果实附生微生物/溢糖性霉斑
Key words
fruit stain/Vitis rotundifolia/pathogen identification/epibiotic microbe of fruit berries/mildew on berry
维普
万方数据