Study of Transcriptional Regulation of Vitis amurensis Rootstock to Improve Cold Tolerance
Grafting with Vitis amurensis as rootstock can improve the cold tolerance of grapevine,but its molecular regulation mechanism is still unknown.In this study,Using heterografted seedlings with Vitis amurensis as rootstock and homografted seedlings of'Pinot Noir'as materials,transcriptome data were used to analyze the differences in gene expression between the two groups under low temperature stress.The results showed that 330 genes were significantly up-regulated and 190 genes were significantly down-regulated in heterografted seedlings.GO enrichment analysis showed that the DEGs between heterografted and homografted seedlings were related to abiotic stress,especially to temperature stress.Among all DEGs,27 heat shock protein genes,three key enzyme genes for the synthesis of osmoregulatiory substances and six key genes of ROS scavenging were significantly up-regulated.And seven gene families including 23 transcription factors,such as WRKY,NAC,MYB,HSF,AP2/ERF,Zinc Finger and MYC were differential expressed,which may play an important role in protecting and regulating the scions under chilling stress.Through the analysis on DEGs between heterografted and homografted seedlings under chilling stress,it will provide a basis for explaining the molecular mechanism of improving the cold tolerance of grapevine heterografted seedlings using vitis amurensis as rootstock.
万方数据
维普
