Construction of Grapevine Genetic Map Based on WGS and Positioning QTL for Resistance to Ripe Rot
Grape ripe rot is a kind of fungal disease which harms ripening fruit and seriously affects grape yield and quality.American grape has characteristics of adapting to humid and hot climate in south area and high resistance to ripe rot.Therefore,it is of great significance to construct a high-density molecular genetic map of American grape,locate its QTL for resistance to ripe rot,and use the disease resistance gene to guide grape disease resistance breeding.In this study,165 self-inbred offsprings of American grape'C30-5-1'were used as the mapping population,based on whole genome resequencing(WGS),the genetic map was constructed,and the ripe rot resistance QTL of grapes was analyzed.The total genetic distance of the constructed grape molecular genetic map was 3138.74 cM,and the average genetic distance between adjacent markers was 0.98 cM,which was evenly distributed and good quality.Based on the offspring ripe rot resistance phenotypic data,a ripe rot resistance-related QTL was detected on the 11th linkage group,named Cgr2,which micropotent QTL explained 5.0％phenotypic variation.After comparison analysis with the grape reference genome,the QTL region contained two candidate resistance genes which encoded UDP glycosidyltransferase and flavonoid 3'-monooxygenase,respectively.They might provide an important role for resistance to ripe rot in grapevine.
万方数据
维普
